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KMID : 0381120210430010011
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Genes and Genomics
2021 Volume.43 No. 1 p.11 ~ p.16
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The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry
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Guo Xiaofan
Xie Zhibing
Zhang Yang
Wang Shouming
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Abstract
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Background: Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-deficient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’.
Objective: CYP714C2 was found to be differentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene.
Methods: In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana.
Results: The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 significantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA1+3 contents of the genetically modified Arabidopsis lines were significantly higher than that in the wild type.
Conclusion: We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry.
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KEYWORD
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CYP450, Gibberellin synthesis, Phytohormone, Wild strawberry, Transformation
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